Analysis of Cutaneous Microbiota of Piglets with Hereditary Melanoma-Reference-Cited by-同舟云学术

Analysis of Cutaneous Microbiota of Piglets with Hereditary Melanoma

Published:2018-12-01 Issue:4 Volume:49 Page:285-290
ISSN:1805-9430
Container-title:Scientia Agriculturae Bohemica
language:en
Short-container-title:

Author:

Švejstil R.¹,Salmonová H.¹,Čížková J.²³

Affiliation:

1. Czech University of Life Sciences Prague , Faculty of Agrobiology, Food and Natural Resources, Department of Microbiology, Nutrition and Dietetics , Prague , Czech Republic

2. Czech University of Life Sciences Prague , Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences , Prague , Czech Republic

3. Laboratory of Applied Proteome Analyses , Institute of Animal Physiology and Genetics , Czech Academy of Sciences , Libechov , Czech Republic

Abstract

Abstract Malignant melanoma may be a life-threatening disease caused by various conditions. Cutaneous bacteria could play a role in melanoma development or regression. The present work aimed to analyze the bacterial species present on the epidermis of piglets with hereditary melanoma. Bacteria isolated by swabs directly from melanomas and healthy epidermis were analysed using MALDI-TOF mass spectrometry. From the total of 290 isolates, 92 were identified, while the extraction by ethanol turned out to be more efficient compared to identification by direct transfer. Staphylococcus sciuri, Staphylococcus cohnii, and Lactococcus lactis were significantly more frequent on healthy skin, whereas Staphylococcus chromogenes, Staphylococcus hyicus, and Enterococcus faecalis have thrived significantly better on melanoma. Overall, the results indicate that the micro-biota of melanoma is different from that of healthy epidermis, so piglet skin bacteria inspections are recommended.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences

Link

https://www.sciendo.com/pdf/10.2478/sab-2018-0035

Reference29 articles.

1. Andresen LO (2005): Production of exfoliative toxin by isolates of Staphylococcus hyicus from different countries. Veterinary Record, 157, 376–378.

2. Andresen LO, Ahrens P, Daugaard L, Bille-Hansen V (2005): Exudative epidermitis in pigs caused by toxigenic Staphylococcus chromogenes. Veterinary microbiology, 105, 291–300. doi: 10.1016/j.vetmic.2004.12.006.10.1016/j.vetmic.2004.12.006

3. Bataille V, de Vries E (2008): Melanoma – Part 1: epidemiology, risk factors, and prevention. BMJ, 337: a2249.

4. Brand A, Singer K, Koehl GE, Kolitzus M, Schoenhammer G, Thiel A, Matos C, Bruss C, Klobuch S, Peter K, Kastenberger M, Bogdan C, Schleicher U, Mackensen A, Ullrich E, Fichtner-Feigl S, Kesselring R, Mack M, Ritter U, Schmid M, Blank C, Dettmer K, Oefner PJ, Hoffmann P, Walenta S, Geissler EK, Pouyssegur J, Villunger A, Steven A, Seliger B, Schreml S, Haferkamp S, Kohl E, Karrer S, Berneburg M, Herr W, Mueller-Klieser W, Renner K, Kreutz M (2016): LDHA-Associated lactic acid production blunts tumor immunosurveillance by T and NK cells. Cell Metabolism, 24, 657–671. doi: 10.1016/j.cmet.2016.08.011.10.1016/j.cmet.2016.08.011

5. Cervinkova M, Kucerova P, Cizkova J (2017): Spontaneous regression of malignant melanoma – is it based on the interplay between host immune system and melanoma antigens? Anticancer Drugs, 28, 819–830. doi: 10.1097/CAD.0000000000000526.10.1097/CAD.0000000000000526

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