Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model-Reference-Cited by-同舟云学术

Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model

Published:2022-12-01 Issue:1 Volume:18 Page:171-179
ISSN:1896-530X
Container-title:Bio-Algorithms and Med-Systems
language:en
Short-container-title:

Author:

Durak-Kozica Martyna¹,Wróbel Andrzej²,Platt Mark³,Stępień Ewa Ł.⁴

Affiliation:

1. Marian Smoluchowski Institute of Physics , Department of Medical Physics, Marian Smoluchowski Institute of Physics Faculty of Physics, Astronomy and Applied Computer Science , Jagiellonian University , Krakow , Poland ; Centre for Theranostics , Jagiellonian University , Kraków , Poland , Phone: +48 12 664 47 62

2. Marian Smoluchowski Institute of Physics , Department of Medical Physics, Marian Smoluchowski Institute of Physics Faculty of Physics, Astronomy and Applied Computer Science , Jagiellonian University , Krakow , Poland ; Centre for Theranostics , Jagiellonian University , Kraków , Poland

3. Department of Chemistry , Loughborough University , Loughborough , United Kingdom

4. Marian Smoluchowski Institute of Physics , Department of Medical Physics, Marian Smoluchowski Institute of Physics Faculty of Physics, Astronomy and Applied Computer Science , Jagiellonian University , Krakow , Poland ; Centre for Theranostics , Jagiellonian University , Kraków , Poland , Phone: +48 12 664 47 62 , Total-Body Jagiellonian-PET Laboratory , Jagiellonian University , Kraków , Poland

Abstract

Abstract Objectives: Extracellular vesicles (EVs) are heterogeneous membrane vesicles in diameter of 30-5000 nm, that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes and apoptotic bodies. The purpose of this study was to compare the distribution of EVs obtained under different conditions of differential centrifugation, including ultracentrifugation, with the results developed based on a theoretical model. Methods: Immortalized endothelial cell line that expresses h-TERT (human telomerase) was used to release of EVs: microvascular TIME. EVs were isolated from the culture medium at different centrifugation parameters. The size distribution of the EVs was measured using TRPS technology on a qNano instrument.

Publisher

Walter de Gruyter GmbH

Subject

Health Informatics,Biochemistry, Genetics and Molecular Biology (miscellaneous),Medicine (miscellaneous),General Computer Science

Link

https://www.sciendo.com/pdf/10.2478/bioal-2022-0088

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