Determination of the binding mechanism of cobalt(II) meso-tetraphenyl porphyrin with plant-esterase-Reference-Cited by-同舟云学术

Determination of the binding mechanism of cobalt(II) meso-tetraphenyl porphyrin with plant-esterase

Published:2021-03-01 Issue:1 Volume:23 Page:25-30
ISSN:1899-4741
Container-title:Polish Journal of Chemical Technology
language:en
Short-container-title:

Author:

Liu Zishan¹²,Dong Liang¹,Li Feifeng¹,Hou Changjun²,He Kun²,Huo Danqun²

Affiliation:

1. Sichuan University of Science & Engineering , Zigong 643000 , China

2. Key Laboratory of Biorheology Science and Technology , Ministry of Education, College of Bioengineering, Chongqing University , Chongqing 400044 , China

Abstract

Abstract Plant-esterase (EC 3.1.1.X) has received much attention because plant esterase and acetylcholinesterase (AChE) share a similar sensitivity towards organophosphorus (OP) pesticides detection with the same inhibition mechanism. To improve the analytical performance, tetraphenyl metal porphyrin, as an indicator was introduced to combine with plant-esterase. The time of reach equilibrium in PBS solution was shortened after adding plant-esterase by assaying the intensify change of the porphyrin spectrum. Meanwhile, intensify of porphyrin spectrum with plant-esterase was increased compared with that of only the porphyrin spectrum in solution. Tetraphenyl metal porphyrin, such as cobalt(II) meso-tetraphenyl porphyrin, is a mixed reversible inhibitor of plant-esterase from kinetic parameters. The combination ratio of plant-esterase and porphyrin is 2:1. On the other hand, the interaction between CoTPPCl and plant-esterase is the strongest among all tested tetraphenyl metal porphyrin. And the mixed system (CoTPPCl-plant-esterase) showed the best sensitivity towards the tested pesticide. All these results indicated that a complex system composed of tetraphenyl metal porphyrin and plant-esterase was fit for detecting pesticides. They make meaningful guidance on the further design of sensing material in monitoring pesticides.

Publisher

Walter de Gruyter GmbH

Subject

General Chemical Engineering,General Chemistry,Biotechnology

Link

https://www.sciendo.com/pdf/10.2478/pjct-2021-0004

Reference12 articles.

1. 1 . Wang, M., Gu, X., Zhang, G., Zhang, D. & Zhu, D. (2009). Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles. Langmuir., 25, 2504–2507. DOI: 10.1021/la803870v.

2. 2. Kulikova, O.M., Ivanova, Y.B. & Mamardashvili NZ. (2011). Determination of acidity of di-, tri-, and tetraazaporphyrins in dimethyl sulfoxide-potassium cryptate medium. Russ. J. Gen. Chem. 81, 602–606. DOI: 10.1134/S1070363211030285.

3. 3. Roales, J., Pedrosa, J.M., Castillero, P., Cano, M. & Richardson, T.H. (2011). Optimization of mixed Langmuir--Blodgett films of a water insoluble porphyrin in a calixarene matrix for optical gas sensing. Thin Solid Films. 519, 2025–2030. DOI: 10.1016/j.tsf.2010.10.038.

4. 4. Whittington, C.L., Maza, W.A., Woodcock, H.L. & Larsen, R.W. (2012). Understanding Ion Sensing in Zn(II) Porphyrins: Spectroscopic and Computational Studies of Nitrite/Nitrate Binding. Inorg. Chem. 51, 4756–4762. DOI: 10.1021/ic300039v.

5. 5. Harmon, H.J. (2008). Role of non-linear optics and multiple photon absorbance in enhancing sensitivity of enzyme-based chemical agent detectors. Methods., 46, 18–24. DOI: 10.1016/j. ymeth.2008.05.003.