Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.-Reference-Cited by-同舟云学术

Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.

Published:2019-12-01 Issue:2 Volume:2 Page:19-24
ISSN:2616-1923
Container-title:Malaysian Journal of Halal Research
language:en
Short-container-title:

Author:

Islam Maidul¹,Sarkar Palash Kumar²,Mohiuddin A.K.M.³,Suzauddula Md.⁴

Affiliation:

1. Department of Biotechnology and Genetic Engineering , Mawlana Bhashani Science and Technology University .

2. Sr. Scientific Officer, Microbial Biotechnology Division, National Institute of Biotechnology , Ganakbari, Savar , Dhaka- 1349 , Bangladesh .

3. Biotechnology and Genetic Engineering Department , Mawlana Bhashani Science and Technology University (MBSTU) , Santosh, Tangail-1902. Bangladesh .

4. Department of Nutrition and Food Engineering , Daffodil International University , Dhaka - 1207 , Bangladesh .

Abstract

Abstract Cellulase is an important enzyme in present-day of industrial biotechnology. The current study is concerned with the production and partial characterization of cellulase enzyme from Bacillus sp. The effect of various fermentation conditions for cellulase production through shake-flask culture was investigated. Maximum enzyme production was obtained after 24 hours of incubation in fermentation medium with pH 3.5 at 35°C under having agitation at 150 rpm while inoculums volume 1% was applied. Enzyme production was 1.91 times higher after optimizing the production condition as compared to the basal media. Cellulase characterization revealed that optimum activity was at pH 5.5 and 50°C for 50 minutes. About 68% of the activity retained after heating the crude enzyme solution at 50°C for 30 minutes. This nature makes cellulase a suitable candidate for current mainstream biomass conversions for sustainable agriculture and industrial processes.

Publisher

Walter de Gruyter GmbH

Subject

General Engineering

Link

https://www.sciendo.com/pdf/10.2478/mjhr-2019-0009

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