Comparative Study on Physicochemical, Antioxidant and Antimicrobial Properties of Propolis Collected from Different Regions of Bulgaria
Author:
Tumbarski Yulian1ORCID, Todorova Mina2ORCID, Topuzova Mariyana3ORCID, Gineva Gabriela1ORCID, Yanakieva Velichka1ORCID, Ivanov Ivan3ORCID, Petkova Nadezhda3ORCID
Affiliation:
1. Department of Microbiology , University of Food Technologies , Plovdiv , Bulgaria 2. Department of Organic Chemistry , Paisii Hilendarski University of Plovdiv , Plovdiv , Bulgaria 3. Department of Organic Chemistry and Inorganic Chemistry , University of Food Technologies , Plovdiv , Bulgaria
Abstract
Abstract
Propolis (bee glue), a valuable biological mixture produced by European honey bees (Apis mellifera L.), is widely used as a remedy in traditional and alternative medicine, as an effective food biopreservative and as a nutritional value enhancer. The present study aimed to investigate eighty propolis samples collected from all twenty-eight districts of Bulgaria in the period of 2020–2022 in order to determine their physicochemical properties as well as their antioxidant and antimicrobial potential. The raw propolis samples exhibited brown or green colour depending on the climatic zone from which they are collected and pH values from 4.82 to 5.87, while the moisture content ranged between 0.98% and 2.97%. The total phenolics content (TPC) and total flavonoids content (TFC) varied from 63.14 mg GAE/g to 737.27 mg GAE/g, and from 29.22 mg QE/g to 234.17 mg QE/g, respectively. The propolis samples demonstrated significant antioxidant potential determined by DPPH and FRAP methods, from 18.56 mM TE/g to 1598.66 mM TE/g and from 82.28 mM TE/g to 1208.81 mM TE/g, respectively, whose values showed a positive correlation (r
2) with the TPC and TFC. The results from the antimicrobial screening revealed that the methanolic propolis extracts in concentration of 20 mg/ml exhibited the highest inhibitory effect on the Gram-positive bacteria such as Micrococcus luteus 2YC-YT, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Listeria monocytogenes NBIMCC 8632 and Listeria innocua ATCC 33090.
Publisher
Walter de Gruyter GmbH
Subject
Insect Science,Plant Science
Reference45 articles.
1. Aboulghazi, A., Touzani. S., Fadil, M., Lyoussi, B. (2022). Physicochemical characterization and in vitro evaluation of the antioxidant and anticandidal activities of Moroccan propolis. Veterinary World, 15(2), 341–349. http://doi.org/10.14202/vetworld.2022.341-349 2. Adaškevičiūtė, V., Kaškonienė, V., Kaškonas, P., Barčauskaitė, K., Maruška, A. (2019). Comparison of Physicochemical Properties of Bee Pollen with Other Bee Products. Biomolecules, 9(12), 819. http://doi.org/10.3390/biom9120819 3. Afrouzan, H., Tahghigh, A., Zakeri, S., Es-haghi, A. (2018). Chemical Composition and Antimicrobial Activities of Iranian Propolis. Iranian Biomedical Journal, 22(1), 50–65. http://doi.org/10.22034/ibj.22.1.50 4. Asem, N., Gapar, N. A. A., Hapit, N. H. A., Omar, E. A. (2019). Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract. Journal of Apicultural Research, 59(4), 437–442. http://doi.org/10.1080/00218839.2019.1684050 5. Bankova, V., Popova, M., Trusheva, B. (2016). New emerging fields of application of propolis. Macedonian Journal of Chemistry and Chemical Engineering, 35(1), 1–11. http://doi.org/10.20450/mjcce.2016.864.
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