Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant-Reference-Cited by-同舟云学术

Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant

Published:2023-01-01 Issue:1 Volume:7 Page:87-95
ISSN:2564-615X
Container-title:The EuroBiotech Journal
language:en
Short-container-title:

Author:

Kang Yangjoo¹,Kim Deuk-Su¹,Kim Kibum¹,Myung Soon-Chul²,Oh Yoo Jin³,Park Sungsu⁴,Hinterdorfer Peter³,Ko Kisung¹

Affiliation:

1. Department of Medicine, College of Medicine , Chung-Ang University , Seoul , Korea

2. Department of Urology, College of Medicine , Chung-Ang University , Seoul , Korea

3. Institute of Biophysics , Johannes Kepler University , Linz , Austria

4. Department of Biomedical Engineering, School of Mechanical Engineering , Sungkyunkwan University , Suwon , Korea

Abstract

Abstract Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.

Publisher

Walter de Gruyter GmbH

Subject

Genetics,Molecular Biology,Biomedical Engineering,Molecular Medicine,Food Science,Biotechnology

Link

https://www.sciendo.com/pdf/10.2478/ebtj-2023-0006

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