Investigation regarding the potential application of grape pomace extracts on in vitro plant growth and development

Author:

Bran Petronela Elena1,Nicuţă Daniela2,Grosu Luminiţa3,Patriciu Oana-Irina3,Alexa Irina-Claudia3

Affiliation:

1. “Vasile Alecsandri” University of Bacău, Doctoral School , 157, Calea Mărăşeşti, 600115, Bacău , Romania

2. Department of Biology, Ecology, and Environmental Protection, Faculty of Sciences , “Vasile Alecsandri” University of Bacău, Faculty of Sciences , 157, Calea Mărăşeşti, 600115, Bacău , Romania

3. Department of Chemical and Food Engineering , “Vasile Alecsandri” University of Bacău, Faculty of Engineering , 157, Calea Mărăşeşti, 600115, Bacău , Romania

Abstract

Abstract The grape pomace hydroalcoholic extracts obtained by two different extraction methods were tested for biostimulatory potential activity for Origanum vulgare L. cultures. The total polyphenols contents of extracts were evaluated by Folin-Ciocalteu method. Characteristics such as: pH, salinity, conductivity and total dissolved solids were determined. FTIR and UV spectra of extracts were also recorded. The effect of grape pomace extracts on growth and development of oregano plant were studied by carrying out in vitro propagation of oregano on Murashige and Skoog basal medium supplemented with different concentrations of grape pomace extracts. Biometric measurements, growth rate and biomass accumulation have been narrowly monitored for all samples and compared to the control sample. The results have shown that the morphogenetic response depends both on the proportion in which basal medium was supplemented and on the extraction method used. A stimulation of growth and development at a low concentration of grape pomace extracts has been noted. The best results were observed in the samples with 1 and 5 % of grape pomace extract obtained by classical extraction.

Publisher

Walter de Gruyter GmbH

Subject

General Medicine

Reference40 articles.

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