Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent-Reference-Cited by-同舟云学术

Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent

Published:2019-04-01 Issue:1 Volume:12 Page:103-107
ISSN:1337-978X
Container-title:Acta Chimica Slovaca
language:en
Short-container-title:

Author:

Ostrihoňová Marta¹,Adamíková Jana¹,Molnár Tomáš¹,Antošová Monika¹,Polakovič Milan¹

Affiliation:

1. Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology , Slovak University of Technology , Radlinského 9, 812 37 Bratislava , Bratislava, Slovakia

Abstract

Abstract This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant. Cation-exchange multimodal adsorbent Capto MMC ImpRes was selected as potential chromatographic separation material. Its equilibrium properties were investigated in batch adsorption experiments. The effect of pH in the range of 5.5—7.5 and NaCl concentration in the range of 0—300 mM on the adsorption of rhEPO and contaminant proteins was examined. Optimal conditions found in these equilibrium experiments were applied to rhEPO adsorption in a chromatographic column. Several experiments were carried out at different elution conditions to optimize the rhEPO yield and selectivity.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/acs-2019-0015

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