New molecular markers involved in immune system homeostasis and hemopoietic organ development are differentially regulated during oocytes in vitro maturation

Author:

Moncrieff Lisa12,Kocherova Ievgeniia3,Bryja Artur3,Kranc Wiesława3,Perek Joanna3,Celichowski Piotr2,Kulus Magdalena4,Kempisty Bartosz2345,Mozdziak Paul6,Jeseta Michal5

Affiliation:

1. School of Medicine, Medical Sciences and Nutrition, University of Aberdeen , Aberdeen , United Kingdom

2. Department of Histology and Embryology, Poznań University of Medical Sciences , Poznań , Poland

3. Department of Anatomy, Poznan University of Medical Sciences , Poznań , Poland

4. Department of Veterinary Surgery, Nicolaus Copernicus University in Toruń , Toruń , Poland

5. Department of Obstetrics and Gynecology, University Hospital and Masaryk University , Brno , Czech Republic

6. Graduate Physiology Program, North Carolina State University , Raleigh , USA

Abstract

Abstract The growth and maturation of the oocyte is a dynamic process which requires a variable supply of hormones, growth factors and energy. These needs are met partially by the surrounding somatic cells and the cumulus-oocyte complex, which communicate bi-directionally via gap junctions. Identifying and analyzing protein expression in the oocyte can provide insight in its development and growth. Further, like bone marrow stem cells, if relevant marker genes are found in oocytes, there is a potential for the oocyte to be manipulated into becoming hemopoietic stem cells. In this study, porcine oocytes were isolated and subjected to microarray analysis to compare the oocyte gene expression in vivo and in vitro maturation (IVM). Genes identified belonged to both ‘hemopoietic or lymphoid organ development’(GO:0048534) and ‘immune system development’ (GO:0002520), and the markers can be used to identify several activities such as cell migration, neurogenesis and proliferation. The following are the identified genes and all were downregulated after IVM to varying degrees: ID2, VEGFA, TGFBR3, INHBA, CDK6, BCL11A, MYO1E, ITGB1, EGR1, NOTCH2, SPTA1, KIT and TPD52. Our results should provide new markers to further investigate oocyte development and growth regulation. Running title: Markers of hemopoietic organ development

Publisher

Walter de Gruyter GmbH

Subject

Cell Biology,Molecular Biology

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