Hemostatic and Histopathological Effects of Local Mineral Zeolite and Tranexamic Acid in Experimental Femoral Artery Bleeding Model

Abstract

Summary Background/Aim: This study aimed to evaluate the effect of local zeolite and tranexamic acid application on hemostasis duration and histopathological changes in the experimental bleeding model, created by puncturing femoral arteries in rats. Material and Methods: A total of 36 Sprague Dawley female rats weighing an average of 240 ± 20 g were used in the study. The three main study groups were the zeolite, zeolite+tranexamic acid, and control groups. Each group was sacrificed on the seventh and fourteenth days of the study, using subgroups for histopathological findings. After piercing the femoral artery of each rat, one gram of the material assigned to the group was applied to the bleeding site after which a 100-gram scale weight was placed on the site for 30 sec intervals, during which temperature was measured. The same sequence of procedures was repeated for the control group, using only standard compression. Statistical analysis was performed using IBM Statistical Package for Social Sciences (SPSS) 15 statistical software. Significance was evaluated at the level of p< 0.05. Results: The bleeding stop time of the control group was significantly longer than the zeolite and zeolite+tranexamic groups (p< 0.05). There was no statistically significant difference between the zeolite and zeolite+tranexamic groups’ bleeding stop times (p> 0.05) or between the mean wound temperatures of the control and zeolite+tranexamic acid groups when bleeding stopped (p> 0.05). Conclusions: The effectiveness of the zeolite group and zeolite+tranexamic acid mixture is more than the control group in ensuring bleeding control. Their efficacy has been clearly observed in providing hemostasis. In addition, it has been determined that zeolite tranexamic acid mixture causes less exothermic reaction than zeolite group. We believe that this new formula should be developed and used to guide new studies.