The Properties of Immobilized Invertase Onto a New Support Material; Poly(Methacrylamide/Maleic Acid) Copolymeric Hydrogel-Reference-Cited by-同舟云学术

The Properties of Immobilized Invertase Onto a New Support Material; Poly(Methacrylamide/Maleic Acid) Copolymeric Hydrogel

Published:2018-12-01 Issue:2 Volume:26 Page:307-328
ISSN:2067-2446
Container-title:Acta Chemica Iasi
language:en
Short-container-title:

Author:

Öztop Hesna Nursevin¹,Çatmaz Fatma Banu¹,Saraydin Dursun²

Affiliation:

1. Department of Biochemistry, Faculty of Science , Cumhuriyet University , Sivas , Turkey

2. Department of Chemistry, Faculty of Science , Cumhuriyet University , Sivas , Turkey

Abstract

Abstract Poly (methacrylamide / maleic acid) PM/MA and poly (methacrylamide) PM hydrogels were prepared aiming to be used as a support for invertase. Spectrophotometric, thermal analysis methods, swelling and diffusion experiments were used for the characterization of hydrogels. The swelling of PM/MA was higher than that of PM in water. The diffusion of water within the hydrogel was found to be non-Fickian. Invertase was immobilized onto PM and PM/MA (samples named PM-I and PM/MA-I respectively). The optimum pH values were found to be; 6.0, 5.0 and 5.5 for free invertase, PM-I and PM/MA-I respectively. The optimum temperature values were found to be 30 °C, 35 °C and 40 °C for free invertase, PM-I and PM/MA-I respectively. The Michaelis constant (Km) and maximum velocity of the enzymes (Vmax) were Km: 11,75 mM, Vmax: 1,95 μmol min−1 for free invertase, Km: 67,24 mM, Vmax: 60,6 μmol min−1 for PM-I and Km: 74,55 mM, Vmax: 18,12 μmol min−1 for PM/MA-I. PM/MA-I showed excellent thermal, operational and storage stability.

Publisher

Editura Universitatii Alexandru Ioan Cuza din Iasi

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