Correlation of the DNA concentration of human samples to electrical bioimpedance measurements: a pilot study
Author:
Hernández-Salinas César A.1, Corzo-Cruz Alejandro12, Sánchez-Monroy Virginia2, Munguía-Cervantes Jacobo E.3, González-Díaz César A.2
Affiliation:
1. Centro Militar de Ciencias de la Salud – Escuela Militar de Graduados de Sanidad , UDEFA, CDMX , México 2. Instituto Politécnico Nacional – Escuela Superior de Medicina , CDMX , México 3. Instituto Politécnico Nacional – Centro de Nanociencias y Micro Nanotecnologías , CDMX , México
Abstract
Abstract
It is necessary to evaluate the total deoxyribonucleic acid (DNA) concentration in gene expression assays. The existing techniques require equipment that is expensive for many labs in developing countries. Portable and inexpensive equipment is needed for easy and economical DNA quantification. Electrical bioimpedance spectroscopy (EBiS) is a non-invasive and inexpensive technique for examining the electrical properties of biological materials. The aim of this study was to explore a potential correlation between the measurement of total DNA extracted from human samples by UV-Vis spectrophotometry and EBiS. Hence, after quantifying the total DNA extracted from each sample by UV-Vis spectroscopy, EBiS was recorded and a possible correlation between the two measurements was analyzed. Considering the bioimpedance phase parameter at 5.24 MHz, a significant correlation was found with total DNA, especially when the concentration was below 100 ng/μL (Spearman coefficient = 0.82, p<0.005). Additional experiments are warranted to confirm these findings.
Publisher
Walter de Gruyter GmbH
Subject
Biomedical Engineering,Biophysics
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