Novel Approach to Investigate the Effect of High-Dose Methylprednisolone on Erythrocyte Morphology: White Light Diffraction Microscopy
Author:
Deniz Cigdem1, Kocahan Ozlem2, Altunan Bengu1, Unal Aysun1
Affiliation:
1. 1 Department of Neurology , Tekirdag Namik Kemal University , Tekirdag , Turkey 2. 2 Department of Physics , Tekirdag Namik Kemal University , Tekirdag , Turkey
Abstract
Abstract
The present study focuses on quantitative phase imaging of erythrocytes with the aim to evaluate the effects of high-dose methylprednisolone (HDMP) on erythrocytes in vivo under physiological conditions in human blood samples. Samples from ten patients, prescribed to be treated with 1000 mg/day intravenous methylprednisolone for 5 days, were analyzed by white light diffraction phase microscopy (WDPM) for quantitative imaging. WDPM, an optical measurement technique, enables single shot measurement and low speckle noise using white light. Quantitative phase imaging performed with this experimental setup allowed the determination of erythrocyte morphology with 9 different parameters. In vivo quantitative analysis of erythrocytes by WDPM, which is a simple and reliable method, shows that HDMP treatment has no significant effect on erythrocyte morphology. With the developing technology, interdisciplinary studies on individuals under treatment should play an important role in elucidating the interaction between steroids and erythrocytes.
Publisher
Walter de Gruyter GmbH
Subject
Instrumentation,Biomedical Engineering,Control and Systems Engineering
Reference34 articles.
1. Unal, A., Kocahan, O., Altunan, B., Gundogdu, A. A., Uyanık, M., Ozder, S. (2021). Quantitative phase imaging of erythrocyte in epilepsy patients. Microscopy Research and Technique, 84 (6), 1172-1180. https://doi.org/10.1002/jemt.23676 2. Zambrano, P., Suwalsky, M., Jemiola-Rzeminska, M., Strzalka, K. (2018). α1-and β-adrenergic antagonist labetalol induces morphological changes in human erythrocytes. Biochemical and Biophysical Research Communications, 503 (1), 209-214. https://doi.org/10.1016/j.bbrc.2018.06.004 3. Zambrano, P., Suwalsky, M., Villena, F., Jemiola-Rzeminska, M., Strzalka, K. (2017). In vitro effects of the anti-Alzheimer drug memantine on the human erythrocyte membrane and molecular models. Biochemical and Biophysical Research Communications, 483 (1), 528-533. https://doi.org/10.1016/j.bbrc.2016.12.111 4. Suwalsky, M., Jemiola-Rzeminska, M., Altamirano, M., Villena, F., Dukes, N., Strzalka, K. (2015). Interactions of the antiviral and antiparkinson agent amantadine with lipid membranes and human erythrocytes. Biophysical Chemistry, 202, 13-20. https://doi.org/10.1016/j.bpc.2015.04.002 5. Lieser, J., Schwedes, C., Walter, M., Langenstein, J., Moritz, A., Bauer, N. (2021). Oxidative damage of canine erythrocytes after treatment with non-steroidal anti-inflammatory drugs. Tierärztliche Praxis, 49 (6), 407-413. https://doi.org/10.1055/a-1623-7506
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