Determination of some drugs used against neurodegenerative disorders by micellar electrokinetic chromatography with running buffer containing SDS

Author:

Traczuk Adam1,Jaglinska Kamila1ORCID,Polak Beata1ORCID

Affiliation:

1. 1 Department of Physical Chemistry , Medical University of Lublin , Poland

Abstract

Abstract In this work, analysis of some drugs used in the treatment of neurodegenerative disorders (sulpiride, olanzapine, carbamazepine, trazodone, clomipramine, and pridinol) was achieved through micellar electrokinetic chromatography (MEKC). The effect of surfactant (sodium dodecylsulphate), acetonitrile, and buffer pH and concentration on the solute retention was also investigated. Successful separation of all compound mixtures was obtained. The method was applied for the quantitative analysis of investigated compounds, and the LOD and LOQ were determined. The LOD values were in the range from 0.0127 mg/mL for clomipramine, to 0.1398 mg/mL for pridinol, while LOQ were in the range 0.0384 mg/mL for clomipramine, to 0.4237 for pridinol. The mode was also applied for the determination of investigated solutes in pharmaceutical prescriptions.

Publisher

Walter de Gruyter GmbH

Subject

Pharmacology,Molecular Biology,General Medicine,Biochemistry

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