Evaluation of Three (3) Techniques in the Diagnosis of Subclinical Mastitis with Antibiotic Resistance of S. aureus in Nigeria

Author:

Anueyiagu Kenneth N.1,Simon Michael1,Torjir Mercy M.1,Kojah Franca1,Madu Victor O.1

Affiliation:

1. Department of Public Health and Epidemiology, Federal College of Animal Health and Production Technology , Vom , Nigeria

Abstract

Abstract This study was aimed at evaluation of two diagnostic techniques and the cultural isolation of Staphylococcus aureus for the testing of subclinical mastitis in lactating cows. A total of 144 milk samples from 36 cows were examined. Ten (10) ml milk samples were aseptically collected from each quarter into labelled sterile universal bottles. The California Mastitis Test (CMT) and the Methylene Blue Reduction Test (MBRT) were carried out on each sample, before cultural isolation (gold standard test) was conducted. Forty eight milk samples were CMT-positive and 60 samples were MBRT-positive, while the gold standard (S. aureus isolation) had 31 positives. The 89.5 % samples of CMT-positive were correctly identified by the test culture. The proportions of samples that tested negative for Subclinical Mastitis (SCM) that did not have the disease, which is a negative predictive value, are 97.9 % and 92.9 % for CMT and MBRT, respectively. The highest total resistance of S. aureus to antibiotics was detected for gentamycin and chloramphenicol (100 %), followed by streptomycin and amoxicillin at 80.6 % and 74.1 %, respectively. The control and monitoring of subclinical mastitis in lactating cows are of great importance to human health. A good management system constitutes the priority in controlling subclinical mastitis in lactating cows.

Publisher

Walter de Gruyter GmbH

Reference26 articles.

1. Abebe, R., Hatiya, H., Abera, M., Megersa, B., Asmare, K., 2016: Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Vet. Res., 12, 270. DOI: 10.1186/s12917-016-0905-3.

2. Anueyiagu, K. N., Ayanbimpe, G., Ikeh, E., 2020: Bovine mastitis due to coliform bacteria, and susceptibility to antibiotics, Nigeria. Int. J. Vet. Sci. Anim. Husb., 6, 1, 054–061.

3. Barrett, D. J., Doherty, M. L., Healy, A. M., 2005: A descriptive epidemiological study of mastitis in 12 Irish dairy herds. Ir. Vet. J., 58, 31. DOI: 10.1186/2046-0481-58-1-31.

4. Bastan, A., Kacar, C., Acar, D. B., Sahin, M., Cengiz, M., 2008: Investigation of the incidence and diagnosis of sub-clinical mastitis in earlier lactation period cows. Turk. J. Vet. Anim. Sci., 32, 2, 119–121.

5. Bradley, A. J., Green, M. J., 2005: Use and interpretation of somatic cell count data in dairy cows. In Practice, 27, 310–315.

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