DNA Damage Detection After Pesticide Exposure
Author:
Baloďanská Sabína1, Galdíková Martina1, Koleničová Simona1, Holečková Beáta1, Schwarzbacherová Viera1, Halušková Jana1
Affiliation:
1. Department of Biology and Physiology , University of Veterinary Medicine and Pharmacy in Kosice , Komenského 73 041 81 , Košice , Slovakia
Abstract
Abstract
Thiacloprid is an insecticide of the neonicotinoid family that acts on the insect’s nAChR receptor and causes its paralysis and subsequent death. The genotoxicity of the insecticide Calypso® 480 SC (with the active substance thiacloprid) to human peripheral lymphocytes was examined in vitro by comet analysis and phosphorylated H2AX, where we detected DNA breaks. To detect DNA damage in comet analysis, we used thiacloprid-based insecticide in three different concentrations (60, 240, and 480 μg.ml−1) during 2 h of exposure. We recorded a statistically significant percentage of damage at a concentration of 240 and 480 μg.ml−1. We monitored the toxicity of thiacloprid using yH2AX foci, and we did not observe a statistically significant number of foci compared to the negative control.
Publisher
Walter de Gruyter GmbH
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