Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria-Reference-Cited by-同舟云学术

Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria

Published:2023-01-01 Issue:1 Volume:7 Page:68-72
ISSN:2543-8050
Container-title:Advanced Research in Life Sciences
language:en
Short-container-title:

Author:

El Ferran Ahlem¹²³,Belkahla Hadjira³,Bouras Noureddine⁴⁵,Atoui Ali⁶,Bakli Mahfoud⁷,Thermoz Jean-Pierre⁸,Chatillon Caroline⁹,Boutoumi Hocine¹⁰

Affiliation:

1. 1 Département de Botanique , Ecole Nationale Supérieure Agronomique d'El-Harrach (ENSA) , Algiers , Algeria .

2. 2 Département de Biologie, Faculté des Sciences de la Nature et de la Vie , Université Blida 1 , Blida , Algeria .

3. 3 Département de Biotechnologies , Laboratoire de Protection et Valorisation des Ressources Agrobiologiques, Faculté des Sciences de la Nature et de la Vie , Université Blida 1, Blida 9000 , Algeria .

4. 4 Laboratoire de Valorisation et Conservation des Ecosystèmes Arides (LVCEA), Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre , Université de Ghardaia , Ghardaïa 47000 , Algeria .

5. 5 Laboratoire de Biologie des Systèmes Microbiens , Ecole Normale Supérieure de Kouba , Alger , Algeria .

6. 6 Laboratory of Microbiology , Department of Life and Earth Sciences, Faculty of Sciences , Lebanese University , Hadath Campus, Beirut P.O. Box 5 , Lebanon .

7. 7 Département de Biologie, Faculté des Sciences et Technologie , Université de Ain Temouchent , Aïn Temouchent , Algeria .

8. 8 Génétique et Écophysiologie de la Qualité des Agrumes, INRA, Centre INRAE/CIRAD - San Giuliano , Corse , France .

9. 9 Qualiplante, Diagnostic Moléculaire des Plantes , Clapiers, Montpellier , France .

10. 10 Laboratoire de Génie Chimique (LGC) , Département de Génie des Procédés, Faculté de Technologie , Université Blida 1 , Blida , Algeria .

Abstract

Abstract Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.

Publisher

Walter de Gruyter GmbH

Subject

General Medicine

Link

https://www.sciendo.com/pdf/10.2478/arls-2023-0008

Reference18 articles.

1. Ghezli, C., & Aouane, B. (2002). Evaluation des maladies des agrumes transmissibles par greffage sur le matériel végétal de multiplication de l'ITAF. Options Méditerranéennes: Série B. Etudes et Recherches(43), 101-104.

2. Ayres, A. J. (2001). Le contrôle des maladies des agrumes au Brésil. Paper presented at the Symposium sur les agrumes, Chine/FAO.

3. Vernière, C., Perrier, X., Dubois, C., Dubois, A., Botella, L., Chabrier, C., Joseph M, B., Vila, N. D. (2004). Citrus viroids: symptom expression and effect on vegetative growth and yield of clementine trees grafted on trifoliate orange. Plant Disease, 88(11), 1189-1197. DOI: 10.1094/PDIS.2004.88.11.1189

4. Durán-Vila, N. (2017). Citrus exocortis viroid Viroids and Satellites (pp. 169-179): Elsevier. DOI: 10.1016/B978-0-12-801498-1.00016-4

5. Sano, T. (2021). Progress in 50 years of viroid research—Molecular structure, pathogenicity, and host adaptation. Proceedings of the Japan Academy, Series B, 97(7), 371-401. DOI: 10.2183/pjab.97.020

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Viroids of the Mediterranean Basin;Viruses;2024-04-15