Effects of dietary <i>Lactobacillus helveticus</i> ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (<i>Oncorhynchus mykiss)</i> under high rearing density-Reference-Cited by-同舟云学术

Effects of dietary Lactobacillus helveticus ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (Oncorhynchus mykiss) under high rearing density

Published:2023-07-01 Issue:3 Volume:23 Page:833-844
ISSN:2300-8733
Container-title:Annals of Animal Science
language:en
Short-container-title:

Author:

Jasim Saade Abdalkareem¹,Altalbawy Farag M.A.²³,Alameri Ameer A.⁴,Ramírez-Coronel Andrés Alexis⁵⁶⁷,Obaid Rasha Fadhel⁸,Al-Hamdani Mais Mazin⁹,Kadhim Abed J.¹⁰,Zabibah Rahman S.¹¹,Alzahrani Hayat Ali¹²,Farsani Sadegh Ghafari¹³,Dadras Mahnaz¹⁴

Affiliation:

1. 1 Medical Laboratory Techniques Department , Al-maarif University College , Al-anbar-Ramadi , Iraq

2. 2 National Institute of Laser Enhanced Sciences (NILES) , Cairo University , Giza , Egypt

3. 3 Department of Chemistry , University College of Duba, Tabuk University , Duba , Saudi Arabia

4. 4 Department of Chemistry , University of Babylon , Babylon , Iraq

5. 5 Health and Behavior Research Group (HBR), Psychometry and Ethology Laboratory , Catholic University of Cuenca , Ecuador

6. 6 University of Palermo , Buenos Aires , Argentina

7. 7 CES University , Colombia

8. 8 Department of Biomedical Engineering , Al-Mustaqbal University College , Babylon , Iraq

9. 9 Pharmaceutics Department, College of Pharmacy , Al-Ayen University , Thi-Qar , Iraq

10. 10 Al-Nisour University College , Iraq

11. 11 Medical Laboratory Technology Department , College of Medical Technology, The Islamic University , Najaf , Iraq

12. 12 Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences , Northern Border University , Arar , Saudi Arabia

13. 13 Isfahan (Khorasgan) Branch , Islamic Azad University , Isfahan , Iran

14. 14 Department of Fisheries, Faculty of Natural Resources , University of Tehran , Karaj , Iran

Abstract

Abstract This study aimed to evaluate the effects of dietary probiotic supplementation of Lactobacillus helveticus on growth, digestive enzymes, and hematological, biochemical, immune, and antioxidant parameters, as well as intestinal microbiota of rainbow trout (Oncorhynchus mykiss). Fish (35.46±0.9 g) were fed with different levels of dietary L. helveticus: control, 1 × 106, 1 × 107, 1 × 108, and 1 × 109 at high stocking density (80 kg m−3) for 60 days. Results indicated that growth performance significantly improved in probiotic supplemented fish (P<0.05). Digestive enzyme parameters revealed that supplementation could significantly increase amylase, protease, and lipase (P<0.05). The treated groups showed significant improvements in serum immune parameters including lysozyme (LYZ), alternative complement (ACH50), respiratory burst activity (RBA), and myeloperoxidase (MPO) (P<0.05). Total protein (TP), albumin (ALB), and globulin (GLO) increased in fish fed experimental diets (P<0.05). Lactate dehydrogenase (LDH) activity was significantly lower in fish fed dietary additives (P<0.05) while white blood cells (WBC), lymphocytes, neutrophils, hematocrit (Hct), red blood cells (RBC) were significantly enhanced (P<0.05). Fish fed with supplemented diets showed significantly enhanced antioxidant status, catalase (CAT) and superoxide dismutase (SOD). Malondialdehyde (MDA) content was significantly lower in fish fed dietary additives (P<0.05). Lactic acid bacteria (LAB) in the treatment groups were significantly increased (P<0.05). In conclusion, dietary supplementation of L. helveticus reduced detrimental effects of high stocking density on growth performance and immune response. It appears that L. helveticus can be recommended as a beneficial probiotic feed additive for rainbow trout.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/aoas-2023-0056

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