Development of Caudal Fin Cell Line from Hill Trout Barilius Bendelisis (Hamilton, 1807) for Cytotoxicity and Transfection Studies
Author:
Kumar Murali Sanjeev1, Soni Pankaj1, Kumar Ravindra1, Singh Neha1, Srivastava Shreya1, Mishra Akhilesh Kumar1, Singh Vijay Kumar1, Kushwaha Basdeo1
Affiliation:
1. ICAR-National Bureau of Fish Genetic Resources , Canal Ring Road, P.O. Dilkusha, Lucknow-226 002 , India
Abstract
Abstract
A cell line named BBdF-1, established from the caudal fin of hill stream fish Barilius bendelisis, has been subcultured for more than 52 passages and is being maintained in L-15 media containing 20% FBS. Species origin of the cell line was confirmed using amplification of partial region of 16S and COI mitochondrial gene sequences. The optimum temperature for growth of BBdF-1 cell line was found to be 28°C. Karyotyping revealed diploid chromosome number as 50. Cells exhibited strong binding for cytokeratin marker and thus were found to be epithelial-like. Strong green fluorescence was observed in BBdF-1 cells transfected with phrGFP-II-N vector, indicating its suitability for utilization in gene expression and manipulation studies. Successful assessment of cytotoxicity of two heavy metals, viz. mercury and chromium, was performed. The cell line can serve as a useful resource material for early toxicity screening of pesticides/pollutant and gene expression.
Publisher
Walter de Gruyter GmbH
Subject
Management, Monitoring, Policy and Law,Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics
Reference42 articles.
1. Avella, M., Berhaut, J., Payan, P. (1994): Primary culture of gill epithelial cells from the sea bass Dicentrarchus labrux. In Vitro Cell Dev Biol Anim, 30, 41-49.10.1007/BF02631417 2. Babich, H., Borenfreund, E. (1987): Cultured fish cells for the ecotoxicity testing of aquatic pollutants. Toxicity Assessment, 2, 2, 119-33.10.1002/tox.2540020202 3. Bakshi, A., Panigrahi, A. K. (2018): A comprehensive review on chromium induced alterations in fresh water Fishes. Toxicol Rep, 5, 440-447.10.1016/j.toxrep.2018.03.007 4. Chaudhary, D. K., Sood, N., Rathore, G., Pradhan, P. K., Punia, P., Agarwal, N. K., Jena, J. K. (2014): Establishment and characterization of macrophage cell line from thymus of Catla catla (Hamilton, 1822). Aquacult Res, 45, 299-311.10.1111/j.1365-2109.2012.03227.x 5. Dewan, S., Singh, Y., Bhatt, J. P. (2016): Proteome adjustments post gradual hypoxic stress in Barilius bendelisis: insights into adaptive strategies of a hill stream cyprinid. Current Proteomics, 13, 1, 20-30.10.2174/1570164613666160413122431
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|