Proinflammatory Cytokine Polymorphisms and Severity of Periodontitis in a Cohort of Bulgarian Patients

Abstract

Abstract In this manuscript we are discussing our research of the presence of single nucleotide polymorphisms (SNPs) of two proinflammatory cytokines – interleukin 17F (IF-17F) and interleukin 23 (IL-23) in a cohort of Bulgarian patients. The aim of the study was to investigate the presence of SNPs of IL-17F and IL-23 and to explore a relation between IL-23 and IL-17F and severity of periodontitis. In the study 83 individuals were included – 62 patients with severe periodontitis and 21 healthy participants. Detailed periodontal status was taken in order to estimate the diagnosis of each participant. A Real-Time PCR for determination of SNP of IL-17 and IL-23 was performed. The data was summarised and processed by the means of statistical analysis. The results showed that when testing individuals for SNP of IL-17F, three genotypes were detected – TT, CT, CC, with prevalence of the TT genotype. In regards to SNP of IL-23, the widespread distribution of GG genotype represents the major share among all individuals with only three representatives with GA genotype. Important tendencies about the severity of the periodontal disease with regards to the SNPs and their simultaneous presence were found. Conclusion: In the present study two proinflammatory cytokine gene polymorphisms were explored and studied in relation to the severity of the periodontal disease. Their distribution among the tested individuals was established and relation to major parameters of the periodontal disease was evaluated.