Changes of enzymatic antioxidant system in the small intestine of rats after the chronic invasion by Hymenolepis diminuta (Cestoda, Hymenolepididae)

Abstract

Abstract The aim of this study was to evaluate changes in the enzymatic antioxidant system in rat small intestine caused by invasion of tapeworms Hymenolepis diminuta. The study material consisted of samples of the rats small intestine after short- (1.5-months) and long-term (1.5-years) larvae invasion of tapeworm H. diminuta. In tissue extracts the concentration of oxidative stress markers (GSH and TBARS) and activity of antioxidant enzymes (SOD, CAT, total GSHPx, SeGSHPx, GST and GSHR) were determined. Changes demonstrated for GSH and TBARS level and the activity of antioxidant enzymes in the small intestine in rats indicate the induction of oxidative stress and weakening of antioxidant defense mechanisms, after both short- and long-term invasion of H. diminuta tapeworms. Observed profile of antioxidant enzymes activity in the small intestine of rats after prolonged exposure to direct or indirect contact with H. diminuta tapeworms points to the adaptation of the definitive host to oxidative stress and defense against parasitic invasions.