Model-based Optimization of Biopolymer Production from Glycerol

Author:

Sharmaa Kavita1,Srivastava Ashok Kumar2,Sreekrishnan T. R.3

Affiliation:

1. Indian Institute of Technology Delhi,Hauz Khas, New Delhi 110016, India

2. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas

3. Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India

Abstract

The present study focuses on sustainable production of biodegradable polymers by <br /> Cupriavidus necator DSMZ 545 using glycerol as substrate. The batch growth and biopolymer production kinetics were established in a 7-L bioreactor, which resulted in a <br /> total biomass of 8.88 g L–1 and poly(3-hydroxybutyrate) (PHB) accumulation of 6.76 g L–1. The batch kinetic and independently acquired substrate inhibition data were then used to develop a mathematical model for PHB production process. This was eventually used to design different nutrient feeding strategies under constant feed rate, decreasing feed rate, and pseudo steady state of substrate (glycerol) to optimize the PHB production during fed-batch cultivation. Among all the fed-batch cultivation strategies, the highest PHB accumulation and productivity of 13.12 g L–1 and 0.27 g L–1 h–1, respectively, was <br /> achieved in fed-batch bioreactor cultivation where a pseudo steady state with respect to <br /> glycerol was maintained.

Publisher

Croatian Society of Chemical Engineers/HDKI

Subject

Process Chemistry and Technology,General Chemistry,Biochemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3