Purification of Hyodeoxycholic Acid and Chenodeoxycholic Acid from Pig Bile Saponification Solution Using Column Chromatography

Abstract

The purpose of this study was to purify hyodeoxycholic acid and chenodeoxycholic <br /> acid from pig bile saponification solution using column chromatography. The optimal <br /> resin was selected through static adsorption experiments. Loading, washing, and elution <br /> conditions were optimized. Hyodeoxycholic acid and chenodeoxycholic acid were further purified through crystallization. The best resin was found to be CG161M macroporous resin. After optimization, the sample was loaded at a volume flow of 3 BV h–1 to adsorb 5 BV, washed with 40 % ethanol, eluted with 45 % ethanol and 60 % ethanol, respectively. This resulted in purities for hyodeoxycholic acid of 70.34 % and 66.21 % for chenodeoxycholic acid with yields of 86.48 % and 90.57 %, respectively. Crystals of hyodeoxycholic acid with a purity of 91.04 % and chenodeoxycholic acid precipitate with a purity of 80.28 % were obtained through crystallization. Hyodeoxycholic acid and chenodeoxycholic acid can be purified using CG161M resin, and subsequent crystallization yields high-purity hyodeoxycholic acid.