RNA dactyloscopy

Abstract

Despite the wealth of data on RNA secondary structure, conformational dynamics and tertiary structure in vitro and in vivo, predicting RNA biological activity in cellular environments remains difficult. Here we describe a method of in silico RNA fingerprinting that allows efficient design of hammerhead ribozyme molecules with a high intracellular efficiency. Our method, which we call RNA dactyloscopy, is a reliable tool for assessing catalytic properties, modeling and design of RNA.