Novel luminescent dyes for confocal laser scanning microscopy used in parasite Trematoda diagnostics*

Abstract

Benzanthrone derivates are now widely used in many industrial and scientific applications as a dye for polymers and textiles. In biochemical, biomedical and diagnostics investigations benzanthrone dyes are used as a lipophilic fluorescent probe as many benzanthrone derivates demonstrate bright fluorescence and they have ability to intercalate between lipids of membrane. The aim of present research was to access the luminescence ability of benzanthrone derivatives using microscopic visualization of biological objects. Accordingly, specimens of freshwater trematode Diplostomum spathaceum, Diplodiscus subclavatus and Prosotocus confusus were stained by all novel benzanthrone dyes using different fixatives. The samples were examined by confocal laser scanning microscope. All dyes showed good results of digestive and reproductive system visualization. Based on obtained results we conclude that all benzanthrone dyes could be used for internal and external structure confocal laser scanning microscopic imaging of trematode specimens.