A rapid method for protein staining in polyacrylamide gels using water saturated with chloroform
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Published:2021-05-11
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ISSN:1734-154X
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Container-title:Acta Biochimica Polonica
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language:
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Short-container-title:Acta Biochim Pol
Author:
Pieta Paweł Piotr,Burchacka Ewa,Śliwa Aleksandra,Szczerba Anna
Abstract
Polyacrylamide gel electrophoresis, followed by an appropriate staining, is a popular and useful analytical procedure for protein identification and characterization. The aim of this study was to develop a method for protein visualization in polyacrylamide gels that would be alternative to Coomassie blue or silver staining. The proposed method is simple, fast and inexpensive. The optimized protocol for protein staining and visualization takes as little as 6 minutes and utilizes deionized water and chloroform. Fluorescence of proteins is induced by UV light and can be detected with a standard transilluminator.
Publisher
Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)
Subject
General Biochemistry, Genetics and Molecular Biology