Properties and Biotechnological Application of Mutant Derivatives of the Mini-Intein PRP8 from Penicillium chrysogenum with Improved Control of C-Terminal Processing
Author:
Publisher
Pleiades Publishing Ltd
Subject
Applied Microbiology and Biotechnology,Biochemistry
Link
http://link.springer.com/content/pdf/10.1134/S0003683820080098.pdf
Reference35 articles.
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2. Novikova, O., Topilina, N., and Belfort, M., Enigmatic distribution, evolution, and function of inteins, J. Biol. Chem., 2014, vol. 289, pp. 14490–14497. https://doi.org/10.1074/jbc.R114.548255
3. Chong, S., Mersha, F.B., Comb, D.G., et al., Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element, Gene, 1997, vol. 192, pp. 271–281. https://doi.org/10.1016/s0378-1119
4. Chong, S., Montello, G.E., Zhang, A., et al., Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step, Nucleic Acids Res., 1998, vol. 26, no. 22, pp. 5109–5115. https://doi.org/10.1093/nar/26.22.5109
5. Mathys, S., Evans, T.C., Jr., Chute, I.C., et al., Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation, Gene, 1999, vol. 231, pp. 1–13. https://doi.org/10.1016/s0378-1119
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