New FRET Pairs of Fluorescent Proteins for In Vitro Caspase Activity Determination
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Published:2022-12
Issue:6
Volume:58
Page:738-743
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ISSN:0003-6838
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Container-title:Applied Biochemistry and Microbiology
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language:en
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Short-container-title:Appl Biochem Microbiol
Author:
Marynich N. K.,Granovsky I. E.,Savitsky A. P.
Abstract
Abstract
A monomeric sensor, TagRFP-23-Ultramarine (TR-23-U), for effector caspase-3 was obtained. The overlap integrals of new pairs of red fluorescent protein TagRFP with four chromoproteins were calculated. The monomeric state of the Ultramarine protein and the TR-23-U sensor was confirmed by gel filtration chromatography and dynamic light scattering. Incubation with caspase-3 showed the possibility of using the new fusion protein as a FRET sensor for apoptosis detection.
Publisher
Pleiades Publishing Ltd
Subject
Applied Microbiology and Biotechnology,Biochemistry
Reference20 articles.
1. Turk, B., Nat. Rev. Drug Discov., 2006, vol. 5, no. 9, pp. 785–799. 2. Mcintosh, A., Meikle, L.M., Ormsby, M.J., Mccormick, B.A., Christie, J.M., Brewer, J.M., Roberts, M., and Wall, D.M., Infect. Immun., 2017, vol. 85, p. e00393-17. 3. Suresh, K., Carino, K., Johnston, L., Servinsky, L., Machamer, C.E., Todd, K., et al., Am. J. Physiol. Lung Cell Mol. Physiol., 2019, vol. 316, pp. 1118–1126. 4. Piston, D.W., Trends Biochem. Sci., 2007, vol. 32, no. 9, pp. 407–414. 5. Rajoria, S., Zhao, L., Intes, X., and Barroso, M., Curr. Mol. Imaging, Bentham Sci. Publ. Ltd., 2014, vol. 3, no. 2, pp. 144–161.
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