Determination of 15 Functional State Biomarkers in Human Urine by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection

Abstract

Abstract The capabilities and limitations of complex procedures for targeted metabolomic analysis using liquid chromatography in combination with tandem mass spectrometry (HPLC-MS/MS) are discussed. An HPLC-MS/MS procedure for the simultaneous determination of the concentrations of 15 biomarkers of the functional state of a human being in urine is presented. The target analytes are biogenic substances of various chemical natures, the basic concentrations of which in biomatrices can vary significantly, up to three orders of magnitude or more. Complex analysis is also difficult to perform due to significant differences in the hydrophilic and hydrophobic properties of the analytes. The testing of the procedure in a bioanalytical experiment made it possible to establish significant differences in the concentrations of a number of biomarkers in the urine of persons with different levels of physical fitness. With a high level of physical fitness, the concentrations of these compounds in urine have lower values in comparison with those in the control group.