99mTc-Novobiocin: a~novel radiotracer for infection imaging

Abstract

Abstract The radiosynthesis of 99mTc-Novobiocin (99mTc-NBN) complex and its suitability as a radiotracer for infection imaging was assessed. The radiochemical purity (RCP) of the 99mTc-NBN complex was determined using radio-TLC and radioactive HPLC and biodistribution was studied in artificially infected (A.I.) rats and rabbit, using single well gamma counter (SWGRC) interface with scalar count rate meter (SCRM) and Gamma Camera (γ-CM). The maximum RCP observed for the preparation having 2 mg of NBN, 111 MBq of sodium pertechnetate (Na99mTcO4) and 125 μL of SnF2 (1 μg/μL in 0.01 N HCl) at a pH 5.6 was 98.97±0.40% and remained stable >90% up to 120 min. The activity of the 99mTc-NBN in the infected muscle (TI) was significantly increased from 6.50±0.15 to 19.00±0.17% and decreased in the inflamed muscle (TII), normal muscle (NT), blood, liver, spleen, stomach and intestine within 120 min. The TI/NT and TII/NT ratios were 7.60±1.08 and 1.60±1.14. The Whole Body Static (WBS) images of A.I. rabbit were obtained at 30, 40, 50 and 60 min after the I.V. administration of 111 MBq of 99mTc-NBN to the A.I. rabbit. The stability in saline and serum, higher TI/NT, lower TII/NT ratios and WBS images confirmed the feasibility of the 99mTc-NBN complex as an infection imaging agent.