Abstract
The aim of the research was to study the behavior of S100-positive cells in the structures of the spleen of laboratory animals in normal conditions and against the background of chronic hypoxic hypoxia. S100-positive cells are professional antigen-presenting cells and at the same time create a microenvironment for B-lymphocytes in the corresponding B-dependent areas of the spleen. Along with this, the expression of markers such as Ki67 and p53 was defined as determining the relationship between the processes of cell regeneration and apoptosis. Chronic isolated normobaric hypoxic hypoxia was simulated on 86 white outbred male rats in special priming chambers with controlled air supply for four hours a day, five days a week for 4 months. The analysis of the data showed that as the duration of the chronic experiment increased, the expression of the p53 marker increased mainly in the B-dependent zones, while the expression of Ki67 in the analogous zones decreased. Also, as the period of chronic hypoxia increased, a significant increase in the expression of the S100 marker in the lymphoid nodules of the white pulp of the spleen was noted. In parallel with this, there is an increase in the relative volume of the T-dependent zone and a decrease in the marginal zone of the white pulp. Thus, an increase in the volume fraction of stromal components along with an increase in the expression of an apoptosis marker in lymphoid nodules of the white pulp of the spleen may indicate the formation of tension in humoral immunity and subsequent depletion of compensatory-adaptive cellular elements of the corresponding zones by the end of the experiment.
Publisher
European Scientific Society