Affiliation:
1. V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Abstract
Background. Atypical ductal hyperplasia is a relatively common breast lesion that increases the risk of breast cancer by 3.5 to 5 times. Genomic rearrangements underlying the development of atypical proliferative lesions and breast cancer lead to gene expression changings.Aim. To determine the mRNA expression profile of neoplasia and oncogenesis driver genes in atypical ductal hyperplasia of the breast.Materials and methods. The real-time reverse transcription polymerase chain reaction was used to explore the expression profile of 46 genes in 107 samples of formalin-fixed paraffin-embedded (FFPE) specimens from atypical ductal hyperplasia, ductal hyperplasia without atypia, ductal carcinoma in situ and normal breast tissue.Results. In atypical ductal hyperplasia, we detected changes in the expression of 22 of 46 studied genes, including ESR1, AR, PRLR, FGFR4, MKI67, CCNB1, KIF14, PAK1, MMP11, GATA3, FOXA1, ZNF703, which were upregulated, and MYC, which was downregulated.Conclusion. The transcriptional signature of atypical ductal hyperplasia was similar to that of ductal carcinoma in situ and breast cancer of luminal subtypes.
Publisher
Publishing House ABV Press
Subject
Pharmacology (medical),Obstetrics and Gynecology,Radiology, Nuclear Medicine and imaging,Oncology,Surgery
Reference21 articles.
1. Simpsons J. WHO Classification of Tumors of the Breast. Lyon: IARC Press 2012. Pp. 88, 89.
2. Rubin E., Visscher D.W., Alexander R.W. et al. Proliferative disease and atypia in biopsies performed for nonpalpable lesions detected mammographically. Cancer 1988;61:2077–82.
3. Liberman L., Cohen M.A., Dershaw D.D. et al. Atypical ductal hyperplasia diagnosed at stereotaxic core biopsy of breast lesions: an indication for surgical biopsy. AJR Am J Roentgenol 1995;164:1111–3.
4. Ioffe O.B., Berg W.A., Silverberg S.G., Kumar D. Mammographichistopathologic correlation of large-core needle biopsies of the breast. Mod Pathol 1998;11:721–7.
5. Friedenreich C., Bryant H., Alexander F. et al. Risk factors for benign proliferative breast disease. Int J Epidemiol 2000;29:637–44. DOI: 10.1093/ije/29.4.637