Rare cases of laboratory tests discrepancies in diagnostics of pediatric Burkitt lymphoma/leukemia-Reference-Cited by-同舟云学术

Rare cases of laboratory tests discrepancies in diagnostics of pediatric Burkitt lymphoma/leukemia

Published:2018-10-27 Issue:3 Volume:13 Page:76-82
ISSN:2413-4023
Container-title:Oncohematology
language:
Short-container-title:Onkogematologiâ

Author:

Demina I. A.¹^ORCID,Illarionova1 O. I.¹^ORCID,Verzhbitskaya T. Yu.²^ORCID,Tsaur G. A.²^ORCID,Rusanova E. B.³,Gorchakova M. V.³,Zueva E. E.⁴,Belogurova M. B.⁵,Uleiskaya G. I.⁶,Shchekina L. A.⁶,Kazakova A. N.¹,Zerkalenkova E. A.¹^ORCID,Olshanskaya Yu. V.¹^ORCID,Abugova Yu. G.¹,Fechina L. G.⁷^ORCID,Miakova N. V.¹^ORCID,Samochatova E. V.¹,Maschan A. A.¹^ORCID,Popov A. M.¹^ORCID

Affiliation:

1. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of Health of Russia.

2. Sverdlovsk Regional Clinical Children’s Hospital No 1; Research Institute of Medical Cell Technologies.

3. I.P. Pavlov First Saint Petersburg State Medical University.

4. I.P. Pavlov First Saint Petersburg State Medical University; Ariel University, Faculty of Natural Sciences, Department of Molecular Biology.

5. St. Petersburg Clinical Scientific and Practical Center of Specialized Medical Assistance (Oncological).

6. City Clinical Hospital No. 31.

7. Sverdlovsk Regional Clinical Children’s Hospital No 1.; Research Institute of Medical Cell Technologies.

Abstract

Introduction. The main features of bone marrow blasts cells in Burkitt lymphoma/leukemia (BL) are L3 morphology, mature immunophenotype of blasts with surface IgM expression, and presence of typical MYC gene rearrangements.The aim of the study was to show discrepancy examples in laboratory signs of BL.Patients and methods. 10 patients (8 boys and 2 girls) aged 1 to 18 years were included in the present study. The inclusion criterion was the identification of discrepancies between flow cytometric, morphological and cytogenetic data.Results. In 2 cases there were no rearrangements of the MYC gene. In 2 patients, the L2 morphological variant went against the presence of typical MYC gene rearrangements. In one case, undifferentiated blasts cells were described by morphology together with presence of surface IgM, and atypical genetics. In 8 patients, there was no expression of surface IgM. Of these, patients with absence of cytomorphological data cytometric and genetic data were controversial.Сonclusion. The cases presented in this study and the cases described in the literature demonstrate the importance of an attentive and comprehensive approach in evaluating the results of laboratory tests in the diagnosis of BL.

Publisher

Publishing House ABV Press

Subject

Oncology,Hematology

Reference23 articles.

1. Burkitt D. A sarcoma involving the jaws in African children. Br J Surg 1958;46(197):218–23. PMID: 13628987.

2. Lenze D., Leoncini L., Hummel M. et al. The different epidemiologic subtypes of Burkitt lymphoma share a homogenous micro RNA profile distinct from diffuse large B-cell lymphoma. Leukemia 2011;25(12):1869–76. DOI: 10.1038/leu.2011.156. PMID: 21701491.

3. Piccaluga P.P., De Falco G., Kustagi M. et al. Gene expression analysis uncovers similarity and differences among Burkitt lymphoma subtypes. Blood 2011;117(13):3596–608. DOI: 10.1182/blood-2010-08-301556. PMID: 21245480.

4. Leoncini L., Raphael M., Stein H. et al. Burkitt lymphoma. In: Swerdlow S.H., Campo E., Harris N.L. et al. (eds). WHO classification of tumours of haematopoietic and lymphoid tissues. IARC press, 2008. P. 262–264.

5. Swerdlow S., Campo E., Harris N.L. et al. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Lyon, France: IARC, 2008;262–4.