Flow cytometric analysis of leukemic blast cells in pediatric B-cell precursor acute lymphoblastic leukemia with translocation t(12;21)(p13;q22)/ETV6-RUNX1-Reference-Cited by-同舟云学术

Flow cytometric analysis of leukemic blast cells in pediatric B-cell precursor acute lymphoblastic leukemia with translocation t(12;21)(p13;q22)/ETV6-RUNX1

Published:2019-01-03 Issue:4 Volume:13 Page:93-103
ISSN:2413-4023
Container-title:Oncohematology
language:
Short-container-title:Onkogematologiâ

Author:

Permikin Zh. V.¹^ORCID,Popov A. M.²^ORCID,Verzhbitskaya T. Yu.³^ORCID,Riger Т. О.³,Arakaev O. R.³,Vlasova A. A.⁴,Olshanskaya Yu. V.²^ORCID,Kazakova A. N.²^ORCID,Tsvirenko S. V.¹^ORCID,Saveliev L. I.⁵^ORCID,Tsaur G. A.⁶^ORCID,Fechina L. G.³^ORCID

Affiliation:

1. Ural State Medical University; Regional Children’s Hospital

2. Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology

3. Regional Children’s Hospital; Research Institute of Medical Cell Technologies

4. Regional Children’s Hospital

5. Ural State Medical University; Regional Children’s Hospital; Research Institute of Medical Cell Technologies

6. Ural State Medical University; Regional Children’s Hospital; Research Institute of Medical Cell Technologies; Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences

Abstract

The objective of the study was searching for surface antigen expression that could predict presence of translocation t(12;21)(p13;q22)/ETV6RUNX1 in pediatric B-cell precursor acute lymphoblastic leukemia patients.Results . ETV6-RUNX1 fusion gene transcript was revealed in 118 (22.4 %) out of 526 children with B-cell precursor acute lymphoblastic leukemia. Leukemic blast cells in ETV6-RUNX1-positive patients more frequently had high CD10 expression, myeloid markers co-expression , including CD13, CD33, CD117, and absence of CD20 than in ETV6-RUNX1-negative ones. Nevertheless diagnostic test performance characteristics of each single parameter was not strong enough for predicting the presence of translocation t(12;21)(p13;q22)/ETV6-RUNX1.Conclusion . Thus application of conventional set of immunological markers does not allow reliable distinguishing this patients’ subgroup. However antibodies panel enlargement, high degree of flow cytometry standardization and additional analytical methods can potentially improve applicability of antigen profile analysis for separation of patients with translocation t(12;21)(p13;q22)/ETV6-RUNX1.

Publisher

Publishing House ABV Press

Subject

Oncology,Hematology

Reference33 articles.

1. Romana S.P., Le Coniat M., Berger R. t(12;21): a new recurrent translocation in acute lymphoblastic leukemia. Genes Chromosomes Cancer 1994;9(3):186–91. PMID: 7515661.

2. Golub T., Barker G., Bohlander S. et al. Fusion of the TEL gene on 12p13 to the AML1 gene on 21q22 in acute lymphoblastic leukemia. Proc Natl Acad Sci USA 1995;92(11):4917–21. PMID: 7761424.

3. Romana S.P., Mauchauffe M., Le Coniat M. et al. The t(12;21) of acute lymphoblastic leukemia results in a telAML1 gene fusion. Blood 1995;85(12):3662–70. PMID: 7780150.

4. Shurtleff S., Buijs A., Behm F. et al. TEL/AML1 fusion resulting from a cryptic t(12;21) is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis. Leukemia 1995;9(12):1985–9. PMID: 8609706.

5. Borkhardt A., Cazzaniga G., Viehmann S. et al. Incidence and clinical relevance of TEL/AML1 fusion genes in children with acute lymphoblastic leukemia enrolled in the German and Italian multicenter therapy trials. Blood 1997;90(2):571–7. PMID: 9226156.

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