A SEARCH FOR CANCER-TESTIS GENES EXPRESSION IN BLOOD AND BIOPSY OF PATIENTS WITH COLORECTAL CANCER

Abstract

CTA are locate in a group of tumor-associated proteins. In normal conditions their expression is limited to embryonic tissues and reproductive tissues of adults. In other tissues CTA are not exist. Interestingly, that the aberrant CTA overexpression is Observed during malignant transformation. In this work we investigated the mRNA of 20 CTA genes overexpression in biopsy and peripheral blood of 49 patients. Among them a MAGE-A1, MAGE-A2 MAGE-A3 MAGE-A4, MAGE-A5, MAGE-A6 mRNAs were detected in same time by using originally primers, that specifically recognize MAGEA-conservative sequences. Similar system of primers was constructed for GAGE1-8 verification. Furthermore, the expression of SSX1, SSX2, SSX4, XAGE1, NY-ESO1 and MAGEC1 was identified. It is found that the detection of mRNA CTA-coding genes can be used as a monitoring test. The presence of mRNA MAGE-C1 and XAGE1 genes in the blood of patients with colorectal cancer could be a potential marker of a more favorable course of the disease. In other hand, the existence of mRNA MAGE-A1-6, GAGE 1-8 and SSX1,2,4 genes may indicate a poor prognosis.