Sperm phenotypes in varicocele

Author:

Zhukov O. B.1ORCID,Bragina E. E.2ORCID,Levin A. V.3ORCID

Affiliation:

1. RUDN University; Association of Vascular Urologists and Reproductologists

2. A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University; N.P. Bochkov Research Centre for Medical Genetics

3. Institute of Toxicology, Federal Medical and Biological Agency of Russia

Abstract

The study objectiveis to microscopically evaluate the morphology of sperm in patients with varicocele after surgical treatment and therapy with Prostatilen® AC.Materials and methods. The treatment group included 20 males between 26 and 45 years of age (mean age 31.6 ± 6.1 years) with various stages of varicocele and fertility problems; among them, 10 patients with subclinical stages of varicocele received conservative treatment with Prostatilen® AC (group 1) and were examined before and after the treatment; 10 males with infertility caused in part by varicocele of the spermatic cord veins were examined 6 months to 3 years after surgical treatment (group 2). Standard clinical and lab tests, sperm analysis, electron microscopy of the ejaculate were performed. The control group included 65 fertile males whose sperm samples were obtained from a bank of reproductive cells and tissues and used for comparison in microscopic examination.Results. In patients who received conservative treatment the number of sperm with immature chromatin decreased (p = 0.045) compared to the control group. This characteristic differed in patients after varicocelectomy and patients after conservative treatment (p = 0.037). Compared to control, the number of sperm with excess residual cytoplasm in the head and neck was higher in patients after varicocelectomy (p = 0.011). After conservative treatment, the number of sperm with excess residual cytoplasm was close to the control number and lower than in patients after varicocelectomy (р = 0.028).Conclusion.In patients with subclinical varicocele, conservative treatment with Prostatilen® AC leads to significant improvement in sperm ultrastructure compared to patients who underwent surgery to treat this pathology. 

Publisher

Publishing House ABV Press

Subject

Urology,Reproductive Medicine,Surgery

Reference43 articles.

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2. Gamidov S.I., Popkov V.M., Shatylko T.V. et al. The role of drug therapy in the management of varicocele. Urologiya = Urology 2018;(5):114–21. (In Russ.).

3. Gamidov S.I., Ovchinnikov R.I., Popova A.Yu. et al. Current approach to therapy for male infertility in patients with varicocele. Terapevticheskiy arkhiv = Therapeutic Archive 2012;84(10):56–61. (In Russ.).

4. Evdokimov V.V., Selivanov T.O. Spermatogenesis disorders in patients with varicocele. Andrologiya i genital’naya khirurgiya = Andrology and Genital Surgery 2006;7(3):12–8. (In Russ.).

5. Zavattaro M., Ceruti C., Motta G. et al. Treating varicocele in 2018: current knowledge and treatment options. J Endocrinol Invest 2018;41(12):1365–75. DOI: 10.1007/s40618-018-0952-7.

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