ZYMOGRAPHIC ANALYSIS OF LIPOLYTICAL ENZYMES OF SUBCELLULAR VIBRIO CHOLERAE FRACTIONS OF O1 AND O139 SEROGROUPS-Reference-Cited by-同舟云学术

ZYMOGRAPHIC ANALYSIS OF LIPOLYTICAL ENZYMES OF SUBCELLULAR VIBRIO CHOLERAE FRACTIONS OF O1 AND O139 SEROGROUPS

Published:2016-04-29 Issue:2 Volume:1 Page:55-59
ISSN:1811-0649
Container-title:Byulleten-Vostochno-Sibirskogo-nauchnogo-tsentra-Sibirskogo-otdeleniya-Rossiyskoy-Akademii-meditsinskih-nauk
language:en
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Author:

�� ,Markov Evgeniy,�� ,Nikolaev Valeriy,�� ,Kozlov Stanislav,�� ,Urbanovich Lyudmila,�� ,Mitkeeva Svetlana

Abstract

Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton �-305 and Triton �- 405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton �- 405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons � preparations of urea extracts from the toxigenic strains (p < 0,05).

Publisher

Infra-M Academic Publishing House

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