POLYMORPHISM OF THE LEPTIN AND DIACYLGLYCEROL-O-ACYLTRANSFERASE GENES IN HOLSTEINIZED BLACK AND WHITE BULLS

Abstract

Allelic polymorphism was studied and the frequency of occurrence of genotypes for leptin (LEP) and diacylglycerol-O-acyltransferase (DGAT1) genes in Black-and-White bulls was determined, their belonging to different genealogical lines of Holstein cattle. Among the animals of the analyzed sample, there was the highest frequency of genotypes LEP CT (53.4 %), DGAT1 AA (50.0 %) and alleles LEP C (0.61), DGAT1 A (0.72). The studied sample of bull breeds by identification to the Holstein breed was structurally represented by the genealogical lines of V. Aidial (77.6%), M. Chiftein (8.6%), R. Sovering and S.T. Rokit (6.9% each). Similar studies of these bulls belonging to different genealogical lines showed that the highest occurrence of genotypes and alleles was LEP CT (57.8%), DGAT1 AA, DGAT1 AK (46.7% each), LEP C (0.60), DGAT1 A (0.70) on the line of V. Aidial, LEP CC, LEP CT (by 50.0%), DGAT1 AA (100.0%), LEP C (0.75), DGAT1 A (1.0) by R. Sovering line, LEP CC, LEP CT (50.0% each), DGAT1 AA (75.0%), LEP C (0.75), DGAT1 A (0.88) by S.T. Rokit, LEP CC, LEP TT (40.0% each), DGAT1 AK (60.0%) on the line of M. Chieftain, respectively. However, it should be noted that according to the M. Chieftain line, the frequency of occurrence of alleles LEP C, LEP T and DGAT1 A, DGAT1 K for the two studied genes was equal and amounted to 0.50. It should also be noted that in the population of bulls with different genotypes for the LEP, DGAT1 genes and unequal linear affiliation to the Holstein breed, the genetic balance is not disturbed.