Evaluation of Multiplex and Uniplex Antibiotic Sensitivity Discs on Bacterial Isolated from Clarias gariepinus from Six Different Ponds in Umuahia

Abstract

The aim of this study was to evaluate the antibiotics types that are more sensitive to bacterial isolated from Clarias gariepinus. Two antibiotics types; namely multiplex and uniplex antibiotic sensitivity disc were used. Disc diffusion technique as described by Kirby-white was employed. Ten antibiotics were used for the study. A simple random sampling method was used. Six fishes were sampled from 6 different ponds, and a total of 36 fishes were screened. Swab samples were inoculated into nutrient agar and incubated at 370C for 24 hours. Grown colonies were sub-cultured into different media, incubated at 370C for 24 hours to obtain pure culture/isolate. Five different bacterial were isolated namely: Staphylococcus spp, Streptococcus spp, Escherichia coli, Salmonella spp and Pseudomonas spp. Pseudomonas spp was more frequently isolated, while Streptococcus spp was the least. Ciprofloxacin (CPX) from multiplex exhibited the highest zone of inhibition at 32mm while Levofloxacin (LEV) exhibited the highest inhibition zones from uniplex disc at 32mm for Stahphylococcus spp. Levofloxain in uniplex exhibited higher zones of inhibition than multiplex disc for Escherichia coli and same is applicable with CPX and LEV for Salmonella spp. The multiplex and uniplex antibiotic sensitivity disc had zone of inhibitions, however, the uniplex exhibited a higher inhibition zone than the multiplex across the five different bacterial isolated. This may be due to good preservation and storage of the antibiotic disc before use.