Comparison of different species identification methods for bacterial HAIs pathogens: biochemical identification, MALDI-TOF, and 16S rRNA

Author:

Korniychuk O.,Sekovska A.,Konechnyi Yu. T.

Abstract

This article investigates the effectiveness and comparative accuracy of three microbial identification methods: biochemical identification, Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF), and 16S rRNA. In total, 309 microbial isolates, representing 54 different species, were analyzed. The results indicated that each method has its own advantages and limitations, but all are capable of providing accurate and reliable identification results, although MALDI-TOF was the fastest, and biochemical identification was the most prevalent.

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