Affiliation:
1. Istituto Sperimentale Italiano Lazzaro Spallanzani, Loc. La Quercia, 26027 Rivolta d’Adda (CR), Italy
Abstract
Aqueous extracts were obtained at low temperature with
the Naviglio technology from grapevine stalks (Merlot), marc (Merlot and
Cabernet Sauvignon) and leaves (Merlot) as typical byproducts of winemaking
industry, and their properties were evaluated cytofluorometrically on human
dermal fibroblasts. Leaf extracts had the greatest total phenolic ((47.6±3.5)
mg/g) and proanthocyanidin ((24.2±0.1) mg/g) contents compared to the others.
The preliminary colorimetric MTT
(3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay
individuated two consecutive non-toxic volume fractions of each extract (from
0.8 to 12.8 %) that were adopted for three cytofluorometric tests. The first
cell membrane test did not evidence any harmful effects against plasma
membranes at the two non-toxic volume fractions. The second mitochondrial
membrane test showed a decreased (p<0.01) percentage of cells ((15.7±8.3) vs
(32.5±1.3) %) with active polarized mitochondrial membranes at the higher
non-cytotoxic volume fractions of extracts from Cabernet Sauvignon marc in
response to 4.5 mM H2O2, and from Merlot stalks
(p<0.05) at 1.5 mM H2O2 ((49.3±6.1) vs
(64.6±2.4) %) and without H2O2 ((89.7±2.4) vs
(96.9±1.8) %), compared to the controls submitted to the same H2O2
concentration. Conversely, mitochondrial activity of leaf extracts
significantly (p<0.05) increased ((96.3±1.8) and (96.4±1.4) %) after
treatment with 0.5 mM H2O2 at both non-cytotoxic
volume fractions compared to control ((88.2±1.1) %). Finally, as evidenced by
the third oxidative status test, stalk extracts did not evidence relevant
effects on the cellular oxidative state, while the extracts of marc and leaves
demonstrated significantly medium (p<0.05) to highly (p<0.001) positive
effects following exposure to H2O2 ranging from 0.5 to
4.5 mM, compared to controls.
Publisher
Faculty of Food Technology and Biotechnology - University of Zagreb
Subject
Industrial and Manufacturing Engineering,General Chemical Engineering,Food Science,Biotechnology
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献