Study of HLA-A and HLA-DR Polymorphism in Local Population, Pakistan

Abstract

The compatibility of human leukocyte antigen (HLA) between donor and recipient is a major threat during kidney transplantation. HLA alleles are highly diverse among different individuals of the same population. HLA genes are responsible for generating immune responses by encoding cell-surface proteins. HLA genes have many different alleles and they are called antigens due to their role in organ transplantation. The current study has performed an experiment for HLA-A and HLA-DR typing through advanced molecular techniques. The 100 different samples from the kidney transplantation section were collected and Sequence-Specific Oligonucleotides (SSO) typing was performed on each sample. The frequencies of HLA-A and HLA-DR were determined on the basis of different perspectives i.e. blood groups, donor and recipient, ethnicity, gender, age groups, and cities. The HLA alleles were differentially frequent among different individuals in Pakistan which showed HLA polymorphism. HLA-A 68 was most frequently observed in this study while HLA-DR alleles have different proportions on the basis of different standpoints. This study has proved that HLA genes show diverse nature and polymorphism to a great extent even in the same population which should be considered crucial during kidney transplantation.