Mouse Skin Bioassay for Chemical Carcinogens

Abstract

The mouse skin initiation/promotion bioassay is one of the proposed bioassays of the Carcinogenesis Testing Matrix for tier II (Bull and Pereira, 1980). A review of the literature indicated that 544 chemicals and substances have been examined by application to mouse skin for carcinogenic activity. Poly-cyclic aromatic hydrocarbons, direct acting alkylating agents, and environmental samples of complex mixtures and subtractions of them that include condensates of automobile exhaust and cigarette smoke have been demonstrated to be carcinogenic by the mouse skin bioassay. Chemical classes of carcinogens that have not been demonstrated to contain initiation and carcinogens in mouse skin include azoxy, diazo, halogenated methanes, hydrazine, inorganics, steroids, and sulfonates. The mouse skin assay can be modified so mat the test substance is administered systemically i.e., oral and intraperitoneal and the promoter applied topically. This modification has the potential of increasing the number of chemical classes detected in the mouse skin initiation/promotion bioassay.