The Effect of RNA Inactivator(s) Synthesized from Cu2+ and Hydroquinone on Monoribonucleotides

Author:

Masoud Asaad N.1,Al-Moslih Moslih I.1,Dubes George R.1

Affiliation:

1. Department of Medical Microbiology University of Nebraska Medical Center Omaha, NE 68105

Abstract

Preparations containing RNA inactivator(s) synthesized from Cu2+ and hydroquinone were tested for their effect on monoribonucleotides. The preparations caused the disappearance of large fractions of all of the monoribonucleotides tested, which were the 3′-ribonucleotides and the 5-ribonucleotides of adenine, cytosine, guanine, and uracil, and an impurity identified as the 2′-ribonucleotide of uracil. Control preparations with Cu2+ but without hydroquinone had no effect on the ribonucleotides. Control preparations with hydroquinone but without deliberately added Cu2+ caused the disappearance of relatively small fractions of the ribonucleotides tested. The activity of these control preparations is probably attributable, at least in part, to trace copper impurity or contamination. The addition of L-histidine protected the ribonucleotides against the effect of the RNA inactivator preparations from Cu2+ and hydroquinone, just as the L-histidine protects naked viral RNA against the RNA inactivator(s). The hypothesis that the ribonucleotide moieties of the viral RNA are the targets of the RNA inactivator(s) is discussed.

Publisher

SAGE Publications

Subject

Toxicology

Reference11 articles.

1. 2. Dubes, G. R., Wegrzyn, R. J., Al-MOSLIH, M. I., and Masoud, A. N. (1979). Abstr. Annu. Meeting Am. Soc. Microbiol. p. 276.

2. Inactivation of transfective poliovirion RNA by a product or products of the interaction of trace copper with an impurity or impurities in reagent-grade phenol

3. Identification of hydroquinone and catechol as impurities in reagent-grade phenol using high performance liquid chromatography with ultraviolet and electrochemical detectors

4. 5. Dubes, G. R., and Masoud, A. N. (1980). Abstr. Ann. Meeting Am. Soc. Microbiol., p. 163.

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