Reverse Transcription Polymerase Chain Reaction (RT-PCR) as a Tool for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Surveillance in the Military

Abstract

Context: Military organizations like civilian communities are under considerable threat due to COVID-19 and other re-emerging infectious diseases, which may impair their operations and training. The aim of this review was to discuss the application of RT-PCR technology for detecting and surveillance of SARS-CoV-2 among armed forces to successfully halt COVID-19 transmission. Evidence Acquisition: Relevant literature was collected from PubMed, Science Direct, and Google Scholar Results: By combining both detection and quantification of pathogens, RT-PCR provides a reliable and sensitive method for detecting SARS-CoV-2 via targeting the viral ORF1ab, E, and N genes. Military surveillance plays an important role in the early detection and identification of positive cases, isolating infected patients, contact tracing, and quarantine to contain the spread of the disease among personnel. This technology emerges as a suitable tool for disease surveillance in the military and early detection and control of diseases. Conclusions: The COVID-19 disease has spread into different parts of the world and may affect military training and operations. So, there is a need for active disease surveillance and preparedness plans in the military. Accordingly, RT-PCR, as a standard tool for SARS-CoV-2 detection, can be used for the surveillance and monitoring of military personnel to successfully curtail COVID-19.