Seroprevalence of Human Fascioliasis Using ELISA in Sistan and Baluchestan Province, Southeast of Iran

Abstract

Background: Fascioliasis is a zoonotic disease caused by the liver parasites, Fasciola hepatica, and F. gigantica. Objectives: The aim of this study was to determine the seroprevalence of fascioliasis in Zahedan and the surrounding towns (i.e., Zabol, Chabahar, Iranshahr, etc.) in the Southeast of Iran. Methods: This cross-sectional study was conducted in 2017. Based on statistical estimations, 251 human serum samples were collected randomly in Zahedan and surrounding towns (Zabol, Chabahar, Iranshahr, and others), then the samples were analyzed using indirect enzyme-linked immunosorbent assay (ELISA) test. A questionnaire was obtained from each individual. Statistical analysis was performed using the chi-square test in SPSS, version 20. Results: Of the 251 samples, the anti-fasciola antibody was detected in 6 (2.40%) samples. There was a significant relationship between the use of local freshwater vegetables and seropositivity to fasciolosis. Conclusions: This study showed that the prevalence of human fascioliasis was higher among women (2.70%) compared to men (0.0%), and raising health levels and health education can reduce the prevalence of human fascioliasis disease.