The Effect of EDTA Buffer and Temperature on DNA Extraction from Teeth for Molecular Forensic Assessment

Abstract

: Using DNA to generate genetic profiles of individuals is an efficient and accurate technique. Achieving the right and net amount of DNA is one of the challenges in this area. Due to tissues destruction after death, it is usually very difficult to achieve proper DNA. So, the use of hard tissues such as bones and teeth as important resources can help in these cases. Accordingly, the use of ion chelating buffers is one of the most important parts of preparing these tissues to extract DNA. In this study, a buffer containing ethylenediaminetetraacetic acid (EDTA) buffer (0.5 mM) and distilled water was used as a control. Different temperatures were also examined. The average concentration of DNA extracted from the sample into ternary sort at a temperature of 55°C, 37°C, 22°C, and 4°C was equal to 19.68 ng/µL, 12.23 ng/µL, 17.19 ng/µL, and 15.06 ng/µL, respectively. For evaluation, sterile distilled water was used instead of buffer, which was equal to 7.9 ng/µL at 55°C. Based on the results of this study, the buffer containing EDTA was found to be suitable for releasing genomic resources from bones and teeth.