Abstract
Background: The ability of anhydrobiotic to survive long periods of stress conditions is associated with high trehalose levels found in these organisms as a responsive mechanism against stress and adverse conditions. Many methods and factors function as determinant agents of trehalose production and preservation in yeasts. Objectives: This study aimed to investigate the effect of three parameters on trehalose synthesis, including temperature, rate of feed, and time of aeration. Methods: To induce trehalose production in baker's yeast, we used an experimental design method in an industrial fermenter at the Khuzestan Yeast Company. The investigation of temperature, rate of feed, and time of aeration parameters in our recipe design were also performed after the cessation of feeding molasses. Results: The findings showed that temperature had the greatest effect on the synthesis of trehalose. Moreover, the interaction of three parameters was significantly effective for the stimulation of trehalose synthesis. We could also make a gas production surge in the dough by increasing the level of trehalose. Conclusions: We conclude that controlling the temperature and taking advantage of the interaction between temperature, rate of feed, and aeration time can help achieve the most effective trehalose synthesis and ensuing gas production in the dough.