Experimental Study of Immunological Activity of Live Plague Vaccine Subjected to Extreme Temperature-Reference-Cited by-同舟云学术

Experimental Study of Immunological Activity of Live Plague Vaccine Subjected to Extreme Temperature

Published:2015-08-20 Issue:4 Volume:14 Page:74-79
ISSN:2073-3046
Container-title:Epidemiology and Vaccine Prevention
language:
Short-container-title:Epidemiology and Vaccine Prevention

Author:

Budyka D. A.,Fisun A. A.,Rakitina E. L.,Abzayeva N. V.

Abstract

The study has resulted in the comparative estimate of the immunological activity of the preparations (LPV) with optimal storage mode and after exposure to extreme temperature (37 ± 1 °С) for 6 days, followed by storage at (4 ± 2 °С). Data obtained during the study indicate an identical immune response. After-heat LPV sample did not reduce the ability to exercise immunological activity during the three-year storage (shelf-life of the drug). When the animals vaccination in this group, total number of T-lymphocytes are increased and signs of humoral immunity are shown earlier. Thus, we can predict more labile conditions on the transportation of live bacterial vaccine without compromising its characteristics.

Publisher

LLC Numicom

Subject

Infectious Diseases,Public Health, Environmental and Occupational Health,Epidemiology

Reference12 articles.

1. Bugorkova S.A., Devdariani Z.L., Schukovskaya T.N., Kutyrev V.V. Historical and modern ideas about the problem of specific prophylaxis of plague. Problems especially dangerous infections. 2013; 3: 63 – 69 (in Russian).

2. Basic requirements for vaccine strains of the plague microbe: guidelines. Moscow: Federal Center gosanepidnadzora the Ministry of Health; 2002 (in Russian).

3. Kutyrev V.V., Yeroshenko G.A., Kukleva L.M., Shavina N.Yu., Vinogradov N.A. Comparative genetic characterization of vaccine strain of Yersinia pestis EV and his alleged «virulent derivatives». Microbiology, epidemiology and immunobiology. 2009; 3: 50 – 59 (in Russian).

4. Vityazeva S.A., Starovojtova T.P., Dubrovin V.I., Golubinsky E.P. Guidelines on the use of cellular composition of immune organs of experimental animals to evaluate the immune adjustment of the organism in the process of formation of immunity to the plague: Irkutsk; 2007 (in Russian).

5. Kravtsov A.L., Shmelkova T.P. Secretory degranulation of neutrophils as a trigger of inflammation and control of the immune response: the role of serial leukocyte proteases and proteolytic activity of receptors. Epidemiology and vaccination. 2011; 1: 79 – 87 (in Russian).