Sargachromenol Attenuates Inflammatory Responses by Regulating NF-κB and Nrf2 Pathways in RAW 264.7 Cells and LPS-treated Mice

Author:

Joung Eun-Ji1,Lee Min-Kyeong1,Lee Minsup2,Gwon Misung1,Shin Taisun3,Ryu Heeyeon1,Jeong Hyeon Hak4,Kim Myeong-Jin1,Van Ji Yun4,Kim Jae-Il1,Choi Jinkyung1,Jung Won-Kyo5,Kim Hyeung-Rak14,Lee Bonggi14ORCID

Affiliation:

1. Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

2. Department of Molecular and Cellular Physiology, Louisiana State University Health Shreveport, Louisiana, United States

3. Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea

4. Department of Smart Green Technology Engineering, Pukyong National University, Busan, Republic of Korea

5. Division of Biomedical Engineering and Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan, Republic of Korea

Abstract

AbstractThis study aims to explore the anti-inflammatory mechanisms of sargachromenol in both RAW 264.7 cells and lipopolysaccharide (LPS)-treated mice, as previous reports have suggested that sargachromenol possesses anti-aging, anti-inflammatory, antioxidant, and neuroprotective properties. Although the precise mechanism behind its anti-inflammatory activity remains unclear, pretreatment with sargachromenol effectively reduced the production of nitric oxide, prostaglandin E2, and interleukin (IL)-1β in LPS-stimulated RAW 264.7 cells by inhibiting cyclooxygenase-2. Moreover, sargachromenol inhibited the activation of nuclear factor-κB (NF-κB) by preventing the degradation of the inhibitor of κB-α (IκB-α) and inhibiting protein kinase B (Akt) phosphorylation in LPS-stimulated cells. We also found that sargachromenol induced the production of heme oxygenase-1 (HO-1) by activating the nuclear transcription factor erythroid-2-related factor 2 (Nrf2). In LPS-treated mice, oral administration of sargachromenol effectively reduced the levels of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α) in the serum, suggesting its ability to suppress the production of inflammatory mediators by inhibiting the Akt/NF-κB pathway and upregulating the Nrf2/HO-1 pathway.

Funder

Korea Institute of Marine Science and Technology promotion

National Research Foundation of Korea

Korea Coast Guard

Publisher

Georg Thieme Verlag KG

Subject

Organic Chemistry,Complementary and alternative medicine,Drug Discovery,Pharmaceutical Science,Pharmacology,Molecular Medicine,Analytical Chemistry

Reference42 articles.

1. Chronic diseases, inflammation, and spices: How are they linked?;A B Kunnumakkara;J Transl Med,2018

2. Atherosclerosis–an inflammatory disease;R Ross;N Engl J Med,1999

3. Cytokines as endogenous pyrogens;C A Dinarello;J Infect Dis,1999

4. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation;J R Vane;Proc Natl Acad Sci U S A,1994

5. Signaling to NF-kappaB;M S Hayden;Genes Dev,2004

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3